Expression of matrix metalloproteinases, tissue inhibitors of metalloproteinases and vascular endothelial growth factor in bovine cutaneous tumors induce by papillomaviruses

PN-III-P1- 1.1- PD-2019-0040 nr PD 50/2020

Ministery of Research, Innovation and Digitalization, CNCS/CCCDI_UEFISCDI

Members

  • Florentina (Bocăneți) DARABAN, DVM, PhD - Project director
  • Professor Mihai MAREȘ, DVM, PhD – Mentor

Summary

Papillomaviruses are small oncogenic DNA, able to infect humans, as well as many species of wild and domestic animals, causing cutaneous and mucosal hyperproliferative lesions, which under the influence of favoring factors may suffer malign transformation. Since the bovine papillomavirus constituted a useful animal model in elucidating the pathogenic and oncogenic mechanisms underlying the cancer development, especially the cervical cancer in women, this research aims to perform an analysis by molecular biology techniques (IHC, PCR assay, zymography, Western Blot) of the matrix metalloproteinase enzyme family, their inhibitors and vascular endothelial factor in bovine cutaneous tumors induced by papillomaviruses. The knowledge of expression of examined MMPs, TIMPs and VEGF would allow us to better understand  the etiopathogenetic mechanism(s) responsible for progression and promotion to bovine fibropapillomas, bovine urothelial tumors and equine sarcoid, all induced by Bovine Papillomaviruses. Cattle affected by urothelial tumors and cutaneous fibropapillomas as well as sarcoid affected equids could be of value to investigate new therapeutic strategies useful in comparative pathology. Therefore, a profound knowledge of this proteolytic system may lead to the development of new strategies for inhibition of MMPs in papillomavirus-induced epithelial neoplasms.

Objectives

2021 – Stage 1

  • Activity 1.1
    Histopathological characterization of bovine epithelial tumours and identification of BPV DNA by PCR assay
  • Activity 1.2
    Immunohistochemically and biochemically analysis of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, their inhibitors TIMP-1, TIMP-2, TIMP-3, VEGF and BPV-E5 protein expression in bovine fibropapillomas and normal skin.
  • Activity 1.3
    Disseminations

2022 – Stage 2

  • Activity 2.1
    Immunohistochemically and biochemically analysis of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, their inhibitors TIMP-1, TIMP-2, TIMP-3, VEGF and BPV-E5 protein expression in bovine fibropapillomas and normal skin.
  • Activity 2.2
    Activation evaluation of active and latent MMP gelatinases by gelatin zymography in vitro.
  • Activity 2.3
    Disseminations

Results

Stage 1

Stage 2

Scientific reports

2021

2022