2008-2011

 

Researches related to the physiological and circadian secretor involvements of the pineal gland in the reproduction status of the laying hens

 

 

Exploratory research project - IDEI

1645 - 1049/2008

 

 

 

 

 

ROMÂNĂ

 

 

ENGLISH

 

 

 

[Abstract]

 

 [Research team]

 

 [Young researchers]

 

 [Goals and activities]

 

 [Achieved results]

 

 [Published articles]

Project title:

Researches related to the physiological and circadian secretor involvements of the pineal gland in the reproduction status of the laying hens

Contract no:

1645 - 1049/2008

Project category:

IDEI, Exploratory research project

Financing:

State Budget-National University Research Council (CNCSIS)

Project value:

1,000,000 lei

Project period:

May 2009-October 2011

Granting authority:

State Budget-National University Research Council (CNCSIS)

Contractor:

“Ion Ionescu de la Brad”University of Agricultural Sciences and Veterinary Medicine Iaşi

 

Project manager:

Prof. univ. dr. Paul Corneliu BOIŞTEANU

 

Project abstract [back to TOP ]

 

Epiphysis plays a controller role of daily and seasonal rhythms in most species; in mammals, melatonin secretor rhythm is controlled by the suprachiasmatic nucleus, through the somatic nervous system ways. In birds, pineal gland contains a circadian self-maintained oscillator and several input channels which synchronize the inner biological clock, inclusively through straight light sensitivity. A few input channels, of physical kind (light, temperature, magnetic field) and of biochemical type (vip, norepinephrine) influencing melatonin releasing, function in vitro, representing an appropriate study model of the circadian biological clock. Melatonin and other indolic hormones synthesis occurs in birds in epiphysis, retina; same biosynthesis pathways exist in lateral eyes, proving that primordial function of melatonin relates to photoreception. Pineal gland of birds comprises a circadian oscillator which plays a main role in temporal organizing of cerebral functions. Secretion of pineal indols occurs under high cyclic rhythms, which seems to be essentially endogenous, modulated by l/d succession and temperature. Circadian clock used by each avian pinealocyte to regulate melatonin synthesis transcription is also daily rhythmically expressed through l/d and d/d cycle, both in vivo and in vitro. In order to adapt organism to external environment conditions, the pineal gland, through its hormones, does not act straightly toward gonads but onto the brain and contributes to cerebral functions adaptation to the environmental conditions, the reproduction activity consisting only a side of this process. Pineal gland, by melatonin synthesis, is in fact a photoperiodic translator, which mediates the reproduction function seasonality. Melatonin involvement within the hypothalamic-hypophyseal–gonadal axis could be proved by high levels associated to low gonadotropins secretion, while decrease in melatonin secretion activates the hypothalamic-hypophyseal–gonadal axis.

 

Research team [back to TOP ]

 

Name

Scientific/didactic title

Hired by

Field of competence

Contact

Paul Corneliu BOIŞTEANU

Prof. dr.

U.S.A.M.V. Iaşi

Animals physiology

Morphophysiological basis of the animal productions

Food chain biosafety

paulb@uaiasi.ro

Ioan VACARU-OPRIŞ Prof. dr. U.S.A.M.V. Iaşi

Poultry husbandry technologies

Poultry productions

Artificial incubation

opris_i@uaiasi.ro

Corneliu COTEA

Prof. dr.

U.S.A.M.V. Iaşi

Animal cytology

Animal histology and embryology

Histochemistry

cvcotea@uaiasi.ro

Marius Giorgi USTUROI

Prof. dr.

U.S.A.M.V. Iaşi

Poultry husbandry technologies

Poultry productions

Artificial incubation

umg@uaiasi.ro

Roxana LAZĂR

Asist. dr.

U.S.A.M.V. Iaşi

Animals physiology

Animals anatomy and histology

lazarrxn@yahoo.com

Răzvan RADU-RUSU

Asist. dr.

U.S.A.M.V. Iaşi

Poultry husbandry technologies

Poultry productions

rprobios@yahoo.com

 

Young researchers involvement degree [back to TOP ]

 

Year

Young researchers

Activities

Tasks accomplishment

Published papers

2009

Asist. Dr. LAZĂR Roxana

Biological material sampling, as required by lab assays

Hematological and biochemical assessments on hens, prior to sexual maturity onset.

Monitoring of microclimate parameters (lighting schedule, light intensity, temperature) and of flock health status (morbidity, mortality).

accomplished

1. Boişteanu P.C., Usturoi M.G., Roxana Lazăr, 2009 - Haematological characterization of reproductive status in laying hens by age. Scientific Papers, Bucureşti, ISSN 1224-429

2010

Asist. Dr. LAZĂR Roxana

Biological material sampling, as required by lab assays

Hematological and biochemical assessments on hens, during laying cycle.

Monitoring of microclimate parameters (lighting schedule, light intensity, temperature) and of flock health status (morbidity, mortality).

accomplished

1. Boişteanu P.C., M.G.Usturoi, Roxana Lazăr, B.V.Avarvarei, 2010 – Haematological Characterization of Reproductive Status at Laying Hens by Age. World Academy of Science Engineering and Technology, ISSN 2070-3724,Tokyo, Japan

2. Boişteanu P.C., M.G.Usturoi, Roxana Lazăr, B.V.Avarvarei, 2010 – Changes of Poultry meat Chemical Composition, in Relationship with, Lighting Schedule. World Academy of Science Engineering and Technology, ISSN 2070-3724, Tokyo, Japan

2009

Asist.dr. RADU-RUSU Răzvan

Monitoring of microclimate parameters (lighting schedule, light intensity, temperature) and of flock health status (morbidity, mortality), prior to laying onset.

accomplished

 

2010

Asist.dr. RADU-RUSU Răzvan

Monitoring of microclimate parameters (lighting schedule, light intensity, temperature) and of flock health status (morbidity, mortality) during laying cycle.

Eggs yield assessment.

accomplished

1. Usturoi, M.G., Boişteanu, P.C., Radu-Rusu, R.M., Pop, I.M., Doliş, M.G. and Usturoi, A., 2010-Productive Response of Certain Laying Hens Hybrids, Reared Within Various Farming Systems. 61th Annual Meeting of the European Association for Animal Production. Book of Abstracts, pg. 251. Wageningen Academic Publishers, The Netherlands. ISBN 978-90-8686-152-1; e-ISBN: 978-90-8686-708-0; ISSN 1382-6077.

 

 

Goals and activities within the project [back to TOP ]

 

Year

Goals

Associated activities

Fulfillment degree

totally

partially

unaccomplished

2009

1

Knowledge of fowl pineal secretor functions prior to sexual maturity reaching

Hematological and biochemical assessments related to metabolic profile establishing.

x

Histological studies on pineal gland and retina.

x

2

Knowledge of fowl reproductive status prior to sexual maturity reaching

Hematological and biochemical assessments.

x

Hormonal assessments.

x

Histological studies on reproductive system.

x

3

Monitoring of technological parameters within poultry houses, prior to flock sexual maturity reaching

Microclimate parameters (lighting schedule, light intensity, temperature).

x

Health status (morbidity, mortality).

x

2010

1

Knowledge of adult fowl pineal secretor functions (during laying cycle)

Hematological and biochemical assessments related to metabolic profile establishing.

x

Histological studies on pineal gland and retina.

x

2

Knowledge of adult fowl reproductive status (during laying cycle)

Hematological and biochemical assessments.

x

Hormonal assessments.

x

Histological studies on reproductive system.

x

3

Monitoring of technological parameters within poultry houses, at adult flock (during laying cycle)

Microclimate parameters (lighting schedule, light intensity, temperature).

x

Health status assessment (morbidity, mortality).

x

Eggs yield evaluation.

x

4

Relationship between fowl light exposure and the functionality of the hypothalamic-hypophyseal–gonadal axis

Assessment of melatonin levels during different light exposure schedules.

x

Histological alterations of the pineal gland, produced by different lighting patterns.

x

Microclimate parameters (lighting schedule, light intensity, temperature).

x

Hormonal assessments.

x

Histological studies on reproductive system.

x

2011

1

Knowledge of adult fowl pineal secretor functions (at the end of laying cycle)

Hematological and biochemical assessments related to metabolic profile establishing.

 

 

Histological studies on pineal gland and retina

 

 

2

Knowledge of adult fowl reproductive status

(at the end of laying cycle)

Hematological and biochemical assessments.

 

 

Hormonal assessments.

 

 

Histological studies on reproductive system.

 

 

3

Monitoring of technological parameters within poultry houses, at adult flock (at the end of laying cycle)

Microclimate parameters (lighting schedule, light intensity, temperature).

 

 

Health status assessment (morbidity, mortality).

 

 

Eggs yield evaluation.

 

 

4

Final analysis of the achieved results

Causal debate of the achieved results.

 

 

Elaboration of the conclusions issued from accomplished researches.

 

 

Final results submission.

 

 

 

 

Achieved results [back to TOP ]

 

* during 2009:  Research report (you need Adobe Reader in order to view file)

* during 2010:  Research report (you need Adobe Reader in order to view file)

 

 

 

Published articles [back to TOP ]

 

 

* during 2010:  Published papers (you need Adobe Reader in order to view file)

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