C. Ivas

STUDIES ON MODIFICATIONS OF THE HEPATIC AND RENAL BIOCHEMICAL PARAMETERS OF RABBITS AFTER BEE VENOM INOCULATION

C. Ivas, Gh. Solcan, Geta Pavel, Ionela Burcă, R. Malancuş

Abstract
The experimental study reported in the present paper was performed on rabbits for making evident the effect of various bee venom doses on dynamics of exploration serum biochemical parameters of hepatic and renal function. On this purpose three experimental groups were chosen and bee venom inoculated by three stings (E1 group), 6 stings (E2 group and 12 stings (E3 group). Prior to inoculation and at time intervals of 2 h, 6 h and 24 h after inoculation blood samples were taken off and submitted to biochemical investigations (ALT, AST, GGT, alkaline phosphatase - ALP, creatinine and urea).
The obtained results made evident a hepatic cytotoxic effect (by ALT and AST increase) noticed 24 h. after inoculation at the E1 group and even after 2-6 h. at the E2 and E3. groups. The serum level of ALP was found to decrease slightly 2 and 6 h.after inoculation for every experimental group while after 24 h a clear increase although below the complete limit of the reference values was noticed with the E2 and E3 groups only. The serum concentration of GGT was found to decrease gradually along the 24 h. interval for every group under study although between physiological limits. As regards the renal biochemical parameters a surprising evolution was noticed - at the E3 group the creatinine decreased below the minimum reference limit 6 h. after inoculation and the urea already increased in the inoculation time was continuously increasing 2h and 6h after inoculation, decreasing to the normal value limits after 24 h at E2 and E3.
In conclusion, the present study made evident an acute and subacute hepatotoxic effect of the bee venom depending on the dose but did not confirm the nefrotoxic effect sustaining on the contrary the theory of the beneficial effect for the homeostasis of the organism metabolic processes.

Key words: bee venom, sting, rabbit, ALT, AST, GGT, ALP, creatinine, urea


CERCETĂRI PRIVIND MODIFICĂRILE PARAMETRILOR BIOCHIMICI HEPATICI ŞI RENALI LA IEPURII DOMESTICI INOCULAŢI CU VENIN DE ALBINE

C. Ivas, Gh. Solcan, Geta Pavel, Ionela Burcă, R. Malancuş

Rezumat
Prezenta lucrare propune un studiu experimental la iepuri pentru a evidentia efectul dozelor variabile de venin de albine asupra dinamicii parametrilor biochimici serici de explorare a functiei hepatice si renale. In acest sens, am constituit 3 loturi experimentale la care am inoculat venin de albine prin 3 īntepaturi (lotul E1), 6 īntepaturi (lotul E2) si 12 īntepaturi (lotul E3), iar īnaintea inocularii si la intervale de timp de 2 ore, 6 ore si 24 de ore dupa inoculare, am recoltat probe de sānge pentru investigatii biochimice (ALT, AST. GGT, fosfataza alcalina- PAL, creatinina si ureea).
Rezultatele au relevat un efect citotoxic hepatic (prin cresterea ALT si AST) evidentiat dupa 24 de ore de la inoculare la lotul E1 si chiar de la 2-6 ore de la inoculare la loturile E2 si E3. Nivelul seric al PAL scade usor la 2 si 6 ore de la inoculare la toate loturile experimentale, dar īnregistreaza o crestere evidenta la 24 de ore de la inoculare doar la loturile E2 si E3, fara a depasi īnsa limita superioara a valorilor de referinta. Concentratia serica a GGT se reduce progresiv īn cele 24 de ore, dar in limite fiziologice, la toate loturile investigate. In privinta parametrilor biochimici renali se constata o evolutie surprinzatoare - la lotul E3 creatinina scade sub limita inferioara de referinta la 6 ore de la inoculare, iar ureea, fiind crescuta īn momentul inocularii, continua sa creasca la 2 si 6 ore de la inoculare, dar scade pāna īn limitele valorilor normale dupa 24 de ore, la E2 si E3.
In concluzie, studiul nostru evidentiaza un efect hepatotoxic acut si subacut al veninului de albine dependent de doza, dar nu confirma efectul nefrotoxic, ci dimpotriva sustine teoria efectului benefic al veninului de albine īn homeostazia proceselor metabolice ale organismului.

Cuvinte cheie: venin de albine, īntepatura, iepure, ALT, AST, GGT, PAL, creatinina, uree.