Ayoub, M. M.


MOTHER'S AGE AND LAMBING TYPE AS INFLUENCIAL FACTORS ON BODY GROWTH AND DEVELOPMENT OF YOUTH SHEEP

Ayoub, M., M., El-Far, A., H., Taha, N., M., Korshom, M., A., Mandour, A., A., Abdel-Hamid, H., S., El-Neweshy, M., S.

Abstract
Objective - The aim of this study was planned to throw the light on the hepatotoxic effects of aflatoxin in duck and to evaluate the effects turmeric (Curcuma longa ground roots) for protection against aflatoxicosis and to explore if they can decrease the aflatoxin production by Aspergillus flavus toxigenic strain on poultry diet. Design - in vitro and in vivo experimentation, malondialdhyde (MDA), Glutathione (GSH) and aflatoxin residues. Results - Aflatoxin (AF) from Aspergillus flavus toxigenic strain was selected as a potent and widely distributed hepatotoxin that induces much health and economic hazards in animal and human. Aspergillus flavus was cultivated on rice to produce the aflatoxin that is used in the present study. Pekin ducklings were allotted to control, aflatoxin treated, turmeric and aflatoxin and turmeric groups. Total proteins, albumin, alanine aminotransferase (ALT) (EC 2.6.1.1) and aspartate aminotransferase (AST) (EC 2.6.1.2), cholesterol and triacylglycerols were measured in serum. The levels of hepatic Malondialdhyde and Glutathione levels and aflatoxin residues were also measured. Conclusions - From the obtained results, it can conclude that aflatoxin has hepatotoxic effects through decrease of total proteins, albumin and glutathione. Moreover, increase ALT, AST, cholesterol, triacylglycerols and lipid peroxidation levels. In addition, aflatoxin induced histopathological changes of liver and residues of aflatoxin were measured. Addition of turmeric to duckling's ration were induced a protective effect against aflatoxicosis. So, we advice to use turmeric as a feed additive in poultry farms.

Key words: Aspergillus flavus; Aflatoxin; Turmeric; Aflatoxin residues


ROLUL PROTECTOR BIOCHIMIC AL UNOR PLANTE MEDICINALE ÎMPOTRIVA AFLATOXICOZEI LA BOBOCII DE RAŢĂ: I. TURMERICUL

Ayoub, M., M., El-Far, A., H., Taha, N., M., Korshom, M., A., Mandour, A., A., Abdel-Hamid, H., S., El-Neweshy, M., S.

Rezumat
Obiectiv – Acest studiu a avut ca scop evidentierea efectelor hepatotoxice ale aflatoxinei la rata si evaluarea efectelor turmericului (Curcuma longa radacini) ca protector împotriva aflaoxicozei si asupra reducerii productiei de aflatoxina de catre tulpina toxigena de Aspergillus flavus din nutretul destinat pasarilor. Design – investigatii in vitro si in vivo pentru Malondialdehida (MDA), Glutation (GSH) si reziduuri de aflatoxina. Rezultate - Aflatoxina (AF) produsa de tulpina toxigena de Aspergillus flavus este cunoscuta ca o hepatotoxina puternica si larg raspândita care produce tulburari de sanatate si pierderi în populatiile de animale si la oameni. Aspergillus flavus a fost cultivata pe substrat de orez, pentru a produce aflatoxina utilizata în prezentul studiu. Bobocii de rata Pekin au lotizati astfel, în functie de componenta hranei: lotul de control, lotul cu aflatoxina în hrana, lotul cu turmeric si aflatoxina în hrana, lotul cu turmeric în hrana. Proteinele totale, albumina, alanin-aminotransferaza (ALT) (EC 2.6.1.1), aspartat-aminotransferaza (AST) (EC 2.6.1.2), colesterolul si trigliceridele au fost determinate în serul sanguin. Nivelurile de Malondialdehida hepatica, Glutation si reziduu aflatoxinic au fost, de asemenea, masurate. Concluzii – Din rezultatele obtinute, se poate concluziona ca aflatoxina are efect hepatotoxic, generând scaderi ale nivelurilor de proteine totale, albumina si glutation. Mai mult, a produs cresterea nivelurilor de ALT, AST, colesterol, triacilgliceroli si peroxidare lipidica. În plus, aflatoxina a produs modificari histopatologice ale ficatului, fiind identificate reziduuri de aflatoxina. Adaosul de turmeric în hrana bobocilor de rata a exercitat un efect protector împotriva aflatoxicozei. În concluzie, se recomanda utilizarea turmericului ca aditiv furajer în fermele avicole.

Cuvinte cheie: Aspergillus flavus; aflatoxina; turmeric; reziduuri de aflatoxina