Vioara Miresan


DETERMINATION OF VITAMIN A IN COW MILK THROUGH HPLC

Vioara Miresan, Camelia Raducu, Aurelia Pece, C. Coroian

Abstract
Absorption spectrum record for vitamin A and absorbency reading at 450 nm was conducted with the help of spectrophotometer. The quantitative analysis of retinol was conducted employing a standard curve of retinol-trans-total solutions (sigma) of concentrations between 10-80 μg/ml.

Key words: milk, cow, vitamin A, HPLC

DETERMINAREA VITAMINEI A DIN LAPTELE DE VACA PRIN HPLC

Vioara Miresan, Camelia Raducu, Aurelia Pece, C. Coroian

Rezumat
Īnregistrarea spectrului de absorbtie pentru vitamin A si citirea absorbantei la 450 nm s-a facut cu ajutorul Spectrofotometrului. Analiza cantitativa a retinolului s-a realizat utilizānd o curba etalon īntocmita cu solutii de retinol-trans-total (Sigma) de concentratii cuprinse īntre 10 si 80 μg/ml.

Cuvinte cheie: lapte, vaca, vitamina A, HPLC