Stela Zamfirescu


RESULTS CONCERNING THE FREEZING PRETABILITY OF BUCK SEMEN AND FECUNDITY AFTER ARTIFICIAL INSEMINATION OF LOCAL GOAT

Stela Zamfirescu, Dorina Nadolu

Abstract

The aim of this study was to evaluate the freezing pretability of semen collected from 38 bucks Alpine and Saanen breeds, imported from Germany(2005). For this reason semen was frozen in Tris and Natrium citrate extenders, besides seminal plasma, using the rapid freezing methodin liquid nitrogen. The results of freezing pretability of semen were valuated by monitoring spermatic and cryobiological index : the fresh semen motility versus freezing-thawing semen motility at 24 h after freezing, motility after Bloom staining of spermatozoa, ultrastructural damages studied by electron microscopy and finally, by direct testing of fecundante capacityof spermatozoa by artificial insemination of goat. The mean values of fresh semen motility were 86.81±0.88% for Alpine and 85±2,24% for Saanen, while after defreezing were 46.34±2.66% for Alpine semen and 56.12±4,02% for Saanen semen. Some ultrastructural damages were observed in freezing-thawing spermatozoa (partial and integral plasmatic membranae loss, nuclear and mithocondrial degenaration). The results were appreciated by „in vivo” testing of frozen semen, using artificial insemination of goats and monitoring the fecundity and prolificacy. So, we obtained a very good fecundity (60%) after artificial insemination of very good motylity of the spermatozoa. All the results showed that buck semen has high pretability to freezing and can be used for artificial insemination. This study followed to find a possibility to preserve the good quality semen and to be distributed in time and space for the amelioration of the Romanian local goats.

Key words: frozen semen, pretability, motility, fecundity, prolificacy

REZULTATE PRIVIND PRETABILITATEA LA CONGELARE A SPERMEI DE TAP SI RATA CONCEPTIEI DUPA INSEMINAREA ARTIFICIALA A CAPRELOR LOCALE

Stela Zamfirescu, Dorina Nadolu

Rezumat

Scopul acestor cercetari a fost evaluarea pretabilitatii la congelare a spermei de la 38 de tapi de rasa Alpina si Saanen, importati din Germania(2005).Sperma s-a congelat in medile Tris si Citrat, fara plasma seminala, folosind metoda de congelare rapida in azot lichid. Rezultatele pretabilitatii la congelare s-au evaluat prin determinarea indicilor spermatici si criobiologici: motilitatea dupa 24 de ore a spermei diluate, comparativ cu motilitatea spermei congelata decongelata, motilitatea stabilita dupa coloratia Bloom, stabilirea modificarilor ultrastructurale ale spermatozoizilor congelati –decongelati prin examen electronomicroscopic si stabilirea fecunditatii dupa inseminarea spermei decongelate la capre cu estrul Indus. Valorile medii ale motilitatii spermei diluate au fost de 86.81±0.88% la rasa Alpina si de 85±2,24% la Saanen, in timp ce, dupa decongelare motilitatea a fost de 46.34±2.66% pentru Alpina si de 56.12±4,02% pentru Saanen .Unele modificari ultrastructurale s-au observat la celulele spermatice congelate( frecvente desprinderi de membrana , partiale sau totale, degenerari nucleare si mitocondriale) .Rezultatele fecunditatii spermei s-au apreciat prin inseminarea artificiala a caprelor cu estrul indus, inregistrand fecunditatea si prolificitatea. Astfel, prin inseminarea a 63 de capre de rasa Carpatina s-a realizat o fecunditate foarte buna, de 60% ; sperma inseminata a avut o motilitate foarte mare. Rezultatele obtinute de noi, indica o foarte buna pretabilitate la congelare a spermei de tap, cu care se obtin fecunditati mari dupa inseminare artificiala. Deoarece, sperma de tap se conserva foarte bine prin congelare, exista posibilitatea infiintarii bancilor de sperma pentru diseminarea in timp si spatiu in scopul ameliorarii productiilor la caprele locale din Romania

Cuvinte cheie: frozen semen, pretability, motility, fecundity, prolificacy