N. Pacala


POSSIBILITIES TO INDUCE TWIN CALVING IN COWS BY EMBRYO-TRANSFER

N. Pacala, I. Bencsik, D. Dronca, Ada Cean, V. Caraba, Alexandra Boleman

Abstract
The aim of our paper was to induce twin gestations in cows, by transferring frozen embryos. The embryos necessary for transfer were obtained from 12 donor cows from Romanian Black Spotted breed, super ovulated with FSH pituitary extract (Foltropin-V). Embryos were no surgically recovered at 7 days after the induced estrus, and those that were in 1 and 2 quality code (after IETS Manual) were frozen. At 11 recipient females we transferred embryos no surgically, one embryo for each uterine horn, 6 females were diagnose as pregnant (54,5%), from which 5 carried the gestation to term. From those, 3 cows had twin calving (60%) and 2 had simple caving (40%). 12 recipient cows received two embryos, by no surgical embryo -transfer, on luteal body ipsilateral uterine horn. 6 females have been diagnose pregnant (60%). From those, 2 cows had twinned calving (33,3%) and 3 cows (66,7%) had simple calving. The twin calving at the females that had one embryo transferred in each uterine horn was higher (60%) comparative to the females that had two embryos transferred into the luteal body ipsilateral uterine horn (33,3%).

Key words: cow, embryotransfer, twin calving

POSIBILITATI DE INDUCERE A GEMELARITATII LA VACI PRIN EMBRIOTRANSFER

N. Pacala, I. Bencsik, D. Dronca, Ada Cean, V. Caraba, Alexandra Boleman

Rezumat
Scopul lucrarii noastre a fost de a obtine gestatii gemelare de la vaci, prin transfer de embrioni congelati. Embrionii necesari transferului au fost obtinuti de la 12 vaci donatoare de rasa Baltata cu negru romāneasca, superovulate cu extract hipofizar de FSH (Foltropin-V). Embrioni au fost recoltati nechirurgical la 7 zile de la estrul indus, iar cei īncadrati īn codul 1 si 2 de calitate (dupa Manualul IETS) au fost congelati. La 11 femele receptoare am transferat nechirurgical cāte un embrion īn fiecare corn uterin, 6 femele au ramas gestante (54,5%), din care 5 vaci au dus gestatiile la termen. Dintre acestea, 3 vaci au avut gestatii gemelare (60%) si 2 vaci gestatii simple (40%). La 12 vaci receptoare am transferat nechirurgical cāte doi embrioni īn cornul uterin ipsilateral corpului galben, 6 femele au fost diagnosticate gestante (60,0%). Dintre acestea, 2 vaci au avut gestatii gemelare (33,3%) si 3 vaci au avut gestatii simple (66,7%). Ratele de gestatie gemelara la femelele la care s-au transferat cāte un embrion īn fiecare corn uterin sunt mai mari (60,0%), comparativ cu femelele la care s-au transferat doi embrioni īn cornul uterin ipsilateral corpului galben (33,3%).

Cuvinte cheie: vaca, embriotransfer, gestatie gemelara