Elena Hriscu (Ursu)


RESULTS ON THE ASSESSMENT OF THE DEGREE MICROBIAL CONTAMINATION OF HATCHING EGGS

Elena Hriscu (Ursu), Irina Elena Ismană (Ciobotaru), M.G. Usturoi

Abstract
    The study was conducted in two hatcheries, where many were taken 160 samples (embryonated eggs are in the 18th day of incubation), half in the warm season and a half in the cold season; in practice, they were collected each 80 samples per season (4 x 20 sample sets of incubation / number, corresponding to 80 samples / season).
    Parameters analyzed were: Salmonella spp., Escherichia coli, Staphylococcus spp., Streptococcus spp. Using national working methods, standardized, effective and accredited RENAR. Detection and isolation of bacterial strains was made on liquid and solid culture media for pre-enrichment, enrichment, isolation and biochemical identification. They were used to confirm and galleries mini-API specific to each category of bacteria partly namely Salmonella spp. and Escherichia coli - ID Galleries 32 E; Staphylococcus spp - ID 32 STAPH galleries and Streptococcus spp - rapid ID 32 Strep.
    From a total of 160 samples analyzed in summer, the two hatcheries, resulted in a 19.37% percentage of contaminated samples.
    In winter I examined all 160 samples of same hatcheries and resulted in a rate of 1.25% of contaminated samples.
    From analyzes revealed that embryonated eggs were contaminated with the same types of bacterial strains, which are Escherichia coli and Staphylococcus spp., incubation of both stations.
    The highest incidence was Escherichia coli strain in a percentage of 13.75%; and Staphylococcus spp strains were found at a rate of 5.62%.

Key words: isolation, identification, bacterial strains embryonated eggs