Yilmaz Ebru

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Yilmaz Ebru, Işik Aytaç Levent, Arslanbay Kemal

   Studies providing trout eggs and enhancing efficiency of fertilization have increased in Turkish trout aquaculture industry recent years. Fertilization methods are important for obtaining better quality offspring and rising living creatures as much as broodstock management and feeding regimes. In this study, four different fertilization methods were tested in order to increase the fertilization ratio and determine the most suitable fertilization method for trout eggs. The experiment was carried out in 4 different treatment groups with 3 replicates for each. In related treatment groups, the fertilization of eggs were performed with actifish solution "AS" (15 ml actifish, 1 L of water), isotonic solution "IS" (7 gr NaCl, 1 L of water), NaCO3 solution (8 gr NaCO3, 1 L of water) and Classical method (CM). At the end of the experiment, the fertilization rates between gropus, eyeing rates and the effects of the solutions on sperm quality were analysed; it was aimed to determeine the most efficient artificial fertilization method for trout eggs. The highest fertilization rates were seen in the group "AS" %95.01±0.47 (p<0,05) while the lowest rates were seen in the group "NaHCO3" %90.72±2.54 (p<0,05). The eyeing rates were resulted in the groups "AS" %87.71±0.36, "CM" %69.94±2.48, "IS" %81.57±1.75 and "NaHCO3" %58.53±5.74 respectively. According to these results, the fertilization method performed with "NaHCO3" was found deficient for the artificial fertilization and eyeing rates of trout eggs; while the method "AS" was found as the most influential method to increase the fertilization of trout eggs.

Key words: Onchorynchus mykiss, trout, artificial insemination, egg, fertilization media