Yuli Andriani

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ASSESSMENT ON COW RUMEN FLUID CELLULOSE-AMYLASE ENZYME ACTIVITY AS AN
ALTERNATIVE SOURCE OF CRUDE FIBER DEGRADING ENZYME IN FISH FEED MATERIALS

Yuli Andriani

Abstract
    A study to assess Cow Rumen Fluid Microorganism Cellulose-Amylase as an Alternative Source of Crude Fiber Degrading Enzyme in Fish Food was performed at the Microbiology Laboratory of the Biology Department Unpad and Chemistry Research Laboratory at the Chemistry Department Unpad. This study was aimed to reveal activities of cellulose and amylase enzymes produced by microorganisms in cow rumen fluid, both in the fresh and crude enzyme extract forms, and to compare them to commercial cellulose and amylase enzymes. The design applied for this study was the descriptive method. The parameter observed at the end of this study was the cellulose and amylase enzyme activities in cow rumen fluid, crude enzyme extract from cow rumen fluid, as well as the commercial cellulose and amylase enzymes. The results showed that the cellulose enzyme activity in cow rumen fluid, crude enzyme extract from cow rumen fluid and commercial cellulose enzyme with Novozymes brand were 2.43, 3.06, and 511.49 U/ml, respectively; whereas, the amylase enzyme activity in cow rumen fluid, crude enzyme extract from cow rumen fluid, and Novozymes commercial amylase enzyme were 3.67, 7.08, and 283,819.62 U/m, respectively.

Key words: cow rumen fluid, crude extract enzyme, amylase and cellulose enzymes