Mioara Costache

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RESEARCH CONSIDERING SPERMIATION ON Polyodon spathula STURGEON SPECIES TOWARDS OPTIMIZING SPAWNING BIOTECHNOLOGY AND CONSERVING BIODIVERSITY

Mioara Costache, Daniela Radu, D. Oprea, M. Costache, Carmen Georgeta Nicolae

Abstract
The paper presents the outcome of research concerning spermiation and sperm quality on Polyodon spathula males hormonally induced by carp pituitary powder and different doses of Nerestin 5A. Sperm volume and spermatozoa density per male and per kg of body weight and sperm motility were appraised. The potential of carp pituitary powder (CPP) and of Nerestin 5A synthetic hormone, at three different doses to stimulate spermiation in paddlefish (Polyodon spathula) was tested. Single injection of the Nerestin 5A at 0.05, 0.1 or 0.15 ml/kg increased the number of spermatozoa per kilogram of body weight by 3.2, 3.2 and 4.5 times compared to the control, but the number of the spermatozoa per kilogram of body weight decreased with CPP (4 mg/kg) by 1.5 times compared to the control. The Nerestin 5A prolonged active spermiation, with numbers of spermatozoa ranging from 6.98 to 1.19 x 109kg-1 of body weight up to 96 hours after treatment. Analysis of variance showed significant influence of experimental batches on volume of sperm per male and per kilogram of body weight, and the total number of spermatozoa per kilogram of body weight, but insignificant influence on the total number of spermatozoa per male. The percentage of motile spermatozoa was not different between experimental batches for sperm collection at different times after injection.

Key words: hormone, spermiation, Polyodon spathula, induced breeding