Soeparna Ibrahim

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THE EFFECT OF CAFFEINE EGG YOLK TRIS SEMEN EXTENDER TO THE QUALITY OF SEPARATION OF GARUT RAM SPERM

Soeparna Ibrahim, Tita Damayanti Lestari, Lies Nurfaidah

Abstract
Study on the effect of caffeine within egg yolk Tris semen extender to the quality of separation of Garut ram sperm has been conducted. The aims of the study were to know the effect of caffeine in egg yolk Tris extender on motility and velocity of the sperm after separation and to find out the optimal dose of caffeine for both parameters. This study used Completely Randomized Design with four treatments on different dose of caffeine (0 mM; 2 mM; 4 mM and 6 mM) and each treatment was repeated five times. Results showed that addition of caffeine into egg yolk Tris extender was significantly affect sperm motility (P<0.01) of Garut ram sperm after separation and the best dose of caffeine was 4 mM to be added into Egg Yolk Tris semen extender to obtain the highest sperm motility and velocity.

Key words: Sperm, Garut ram, sperm separation, egg yolk Tris, caffeine