EFFECTS OF USING A DETOXIFYING AGENT IN MIXED FEED, ON THE HAEMATOLOGICAL, IMMUNOLOGICAL PARAMETERS AND HISTOPATOLOGICAL ALTERATIONS IN CHICKEN BROILERS
R. Marţi, I. Vacaru-Opriş, Cristina Gabriela Radu-Rusu, H. Sărăndan
Fight against mycotoxicosis could be carried on through prophylaxis measures, such as well drying the cereals presenting high humidity values, prior to storage. If they are already contaminated with mycotoxins, they could be annihilated through absorption inhibition or by usage of some bacteria which consume mycotoxins. The purpose of this paper was to assess the effect of ochratoxine A (OTA) and deoxynivalenol (DON) on the haematological, immunological an histological response of the chicken broilers, protected or not protected against mycotoxins through feeding or not a detoxifying-mycotoxin inhibitor additive, meaning Mycofix MTV product, from Biomin, at dietary inclusion rates of 1‰ and 3 ‰. The biological material was represented by 111678 ROSS-308 chicken broilers, reared industrially, in deep litter system, till slaughter at 40 days old. Prior to slaughtering, blood samples were taken; digesta samples and internal organ tissues were sampled from slaughtered broilers, in order to assess the influence of the used feed additive on the following parameters: concentration of DON in digesta, concentration of OTA in gall, white blood cells formula (%), properdine (µg/ml), serum lysosime (µg/ml), histopatological alterations in ingluvies, ileum, caecum, liver, kidneys and spleen. Mycotoxicological exam revealed rapid inactivation of DON, since the ingluvies, with: 55.52% at 1‰ Mycofix MTV and 64.41% at 3‰ Mycofix MTV. In caecum, compared to control group, DON decreased by: 11.12 at 1‰ Mycofix MTV and 30.56% at 3‰ Mycofix MTV. Histopatologically, in ingluvies, 3ɠ Mycofix MTV, mucosa lesions were reduced. In ileum and caecum, necrosis, ulcers and flattening of villus drawing supported the protective effect of Mycofix MTV against DON, no matter the dietary dosage. Mycofix MTV diminished the liver excretion of OTA, through gall, with: 15.93% at 1‰ Mycofix MTV and 21.24% at 3‰ Mycofix MTV, as a consequence of OTA inactivation and detoxification in the digestive tract.
Key words: broilers, mycotoxins, properdine, lysosime, histopatology