BIOCHEMICAL DETERMINATIONS AND OXIDATIVE STRESS EVALUATION ON ONCORHYNCHUS MYKISS GROWN IN RECIRCULATING SYSTEM
Mariana Lupoae, V. Cristea, D. Coprean, Mirela Mocanu, Tanţi Patriche, Elena Bocioc
The paper presents the results of the oxidative stress evaluation on rainbow trout (Oncorhynchus mykiss, Fam. Salmonidae) through measures of the lipid peroxidation indice (MDA), total antioxidant capacity (TAC) and analysis of the glucose content (GLU), total protein (PT) and immunoglobulin M(IgM) from blood plasma. The purpose of the experiment consist in the technological performance of the growth on Oncorhynchus mykiss in the biosecurised recirculation system with different stocking density. The biological material was represented by immature rainbow trout with 80±10 g body weight, means ± SEM. The used methods for the TAC and MDA analysis was performed by the spectrophotometric technique and the biochemical samples were read at the Vitros TP and Imola devices. Biochemical indices are in comparison limits with the speciality literature: GLU=69,5÷124 mg/dl; PT=3,45÷4,3g/dl; IgM=49,72÷87,72mg/dl. The MDA concentration has the highest value in L1(5,64nmol/ml plasma) at a value of 42,72% echiv. Trolox TAC. The changes the two indices expect from the oxidative stress (MDA and TAC) and the analysed biochemical indices offer informations about stocking density knowing that the ontogenetic level of the rainbow trout grown in the recirculation system.
Key words: oxidative stress, lipidic peroxidation, rainbow trout, recirculation system