STUDY ON THE DYNAMICS OF SOME BIOACTIVE ELEMENTS FROM BEE POLLEN EXTRACTS
A.-Al. Prelipcean, Otilia Bobiş, St. Lazăr
Analyses carried out have followed the determination of the amount of total polyphenols, flavanols, total flavonoids and antioxidant activity of bee pollen extracts harvested by collectors from Moldova. These determinations were performed according to different conditions: time, temperature and light. Two methanolic extracts have been used different in the methanolic solvent concentration used: 96% and 80%. Analyses were performed on fresh pollen extract, kept a week and kept two weeks, at 4°C, 20°C, the presence or absence of natural light. We have detected fluctuations in the quantities of compounds followed, both in relation to storage conditions and depending on the solvent used. The amounts of total polyphenols detected had mean values between 27.13 and 29.90 mg GAE / g pollen for extract 1 (solvent methanol concentration 96%) and between 23.56 and 27.67 mg GAE / g pollen for extract 2 (solvent methanol concentration 80%). Quantities of flavanols values were between 8.93 and 7.43 mg QE / g pollen (extract 1), and between 4.95 and 5.92 mg QE / g pollen (extract 2), describing a general downward dynamic. Total flavonoids recorded were between 20.44 and 28.00 mg QE / g pollen (extract 1), and between 16.17 and 19.50 mg QE / g pollen (extract 2). The antioxidant activity determined was between 24.18 and 14.24% inhibition (extract 1), and between 26.71 and 19.90% inhibition (extract 2).
Key words: pollen extract, dynamic, polyphenols, flavonoids, antioxidant activity