Siti Darodjah Rasad


MICROSATELLITE ANALYSIS IN BIOTECHNOLGY OF ANIMAL REPRODUCTION

Siti Darodjah Rasad

Abstract
In year 1970 was found nucleotide sequenz which have repeated sequenz of nucleotide. with high polymorh and using PCR could be amplified. That sequenz of nucleotide called Microsatelite. Microsatelite consist of 1 6 repeated nucleotide, which is CA repeated as mostly a repeated DNA in the animal [39]. Based on difference of long and amount ofrepeated nucleotide, there are three kind of DNA satelite, midi-, mini- and microsatelite (Tautz şi Renz, 1984). Microsatelite analysis was used to analyse of paternity and identity of animal, which was done as a conventional analysis with blood group analysis. The advantage of microsatelite analysis compare to blood group system are the exclution probability was high (EXP 99.9%), needs small sampel (tissues, sperm or follicel of hair), could be use for all animal without special age and possible for died animal.

Key words: microsatelite analysis, paternity, reproductive biotechnolgy