Mihaela Oana Arteni

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RESEARCHES ON THE ACTIVITY OF OXIDOREDUCTASES FROM TISSUES SAMPLED IN DIFFERENT STAGES OF DEVELOPMENT AT SILURUS GLANIS

Mihaela Oana Arteni, Z. Olteanu, L. Oprică, M. Bălanu

Abstract
The aim of our research is to study the dynamics of the enzymes activity involved in oxidative stress (superoxide dismutase, catalase and peroxidase) performed on two types of tissues, from muscle and intestine, collected from Silurus glanis aged one, two, respectively three summers. Research has been conducted on four individuals for each age; fish were collected from Fish Farm Ţigănaşi, Iasi County. Superoxide dismutase activity was determined using Winterbourne, Hawkins, Brian and Carrell methods adapted by Artenie, catalase activity was determined using Sinha method, peroxidase was determined using colorimetric method with o-dianisidine and soluble proteins were determined using Bradford method. Data analyses showed that enzyme activities present differences tissue-dependent and also related with the individual age. Oxidoreductases activity is higher in intestinal tissue than in muscle tissue for all three stages of development taken into study. It was also considered in sthe study the type of food used to feed fish at different ages.

Key words: Silurus glanis, superoxide dismutase, catalase, peroxidase, age