RAPD TECHNIQUE USED IN ANALYZING THE GENETIC STRUCTURE OF CYPRINUS CARPIO SPECIES – GALITIAN AND LAUSITZ VARIETIES
R.G. Oroian, T.E. Oroian, Crina Teodora Carsai, Viorica Cosier, L. Sasca
The purpose of this study is to analyze the genetic structure of Cyprinus carpio species, Galitian and Lausitz varieties, from Arinis fishery, Maramures county, using RAPD technique. This work perfomed to improve the artificial reproduction, by making the pairing management using DNA fingerprinting. This manual labor was applied for all the reproduction individuals from the enlarged selection nucleus (150 individuals, 60 from each variety), with the ages between three and six summers. We present in the paper the data from 6 reproduction individuals used for reproduction in 2008. We gathered the biological samples from fish fins and scales, extracted the DNA, using phenol-chloroform method, verified the DNA purity and applied RAPD technique. From a total number of 50 RAPD primers used, only 3 from Operon series (OPM-14, OPM-12 and OPQ-09) allowed the observation of electrophoretic profiles of the amplified samples. We also made the similarity matrix of the two varieties, considering the line number, calculated based on Nei Lee-Dice similarity coefficient. Based on the existent similarity, we performed the UPGMA type dendrogram, represented by two base clusters, where are included the two varieties’ genotypes.
Key words: Cyprinus carpio, RAPD, DNA, dendrogram