CHANGES INDUCED BY SOME NUTRITIONAL FACTORS ON FEMUR HISTOLOGICAL STRUCTURE IN PIG
Gabi Dumitrescu, Lavinia Stef, D. Drinceanu, Liliana Petculescu Ciochina, D. Stef, D. Dronca, Rodica Criste, Liliana Boca, C. Julean
The problems approached by our team are represented by the involvement of different sources and levels of calcium, namely calcium carbonate, fructoborate and alfalfa, in the mineralization of bone tissue. So, we made fix histological preparations of tissue fragments taken from femure, from 9 pigs belonging to three batches: the control batch, where calcium was provided in a proportion of 1% through calcium carbonate, experimental batch 1, where calcium was provided in a proportion of 1.04% through fructoborate, on a calcium carbonate support, and experimental batch 2, where calcium was provided in a proportion of 1.13% through fructoborate + alfalfa, on a calcium carbonate support. The histomorphometric parameters assessed were represented by the volume of bone trabeculae (BV/TV, %) or the percentage of bone tissue in a given volume and the mean width of bone trabeculae. At the same time, in order to establish fructoborate and alfalfa implication in bone mineralization, we supervised the presence and activity of osteoblasts respectively osteoclasts. In the case of the experimental batch 1, the histomorphometric study shows an increase of bone trabeculae dimension, with a mean width of about 115.4μ μ, and also an increase of their mean volume, which is about 36.46%. The trabecular system is dense and present mineralised and ossified territory where are formed by osteoclasts with osteocytes. Peritrabeculary are presented active osteoblasts which are involved in plurilamellar stratification by deposition of young collagen. In the case of individuals from experimental batch 2, trabeculae mean width is about 111,5μ, while their volume is 33.60%.
Key words: pig, bone, fructoborate, calcium, histomorphometry