THE INTERACTION EFFECTS OF SARDINES OIL AND ZINC ON CONCENTRATIONS OF PROSTAGLANDIN-E₂, SERUM ZINC LEVELS,
ZINC ABSORPTION AND ALKALINE PHOSPHATASE ACTIVITY, OF RAM FED WITH BASAL RATION OF SUGAR CANE PULP SILAGE

Diding Latipudin, Soeharsono, Roostita Balia, Winugroho

Abstract
    The purpose of this study was if is any interaction between the sardines oil with zinc on concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption, alkaline phosphatase activity, ram fed the basal ration of sugar cane pulp that have been fermented and urea added. (2) To what extent the effect of the interaction between sardines oil and zinc to concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption, alkaline phosphatase activity, rams basal ration fed sugar cane pulp that have been fermented and urea added. (3.) At the level of zinc and sardines oil how best to concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption and alkaline phosphatase activity, in which rams were given rations of sugar cane pulp that have been fermented and urea added. Most of the experiments conducted in the cage experiment Test Farm, Laboratory of Physiology and Biochemistry and at Livestock Research Center (Balitnak) Ciawi Bogor. Experiments carried out in two stages, namely 1) an experiment in vitro to explore the level of use of urea. Fermented sugar cane pulp using rumen fluid plus buffer fluid compared to fermentation with the bacteria Cytophaga sp. Performed with completely randomized design (CRD) with four levels of Urea (0, 0.5 1.0 and 1.5%) as a treatment and five replications. 2) experiment in vivo to explore the level sardines oil supplementation and zinc. Conducted by randomized block design (RBD). A factor is a supplement of zinc (ZnSO₄) with stage 0, 50 and 100 mg.kg^-1 DM rations, factor B is the sardines oil level with 0, 1.5 and 3% kg^-1 DM rations. Sheep divided into 3 groups based on body weight. Measured variables in addition to the usual parameters used to evaluate rations are also measuring the amount of bacteria and protozoa, VFA concentration, the concentration of N-ammonia rumen fluid. Also measured concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption, and alkaline phosphatase activity. Experiments in vivo performed using rams age 5-7 months weighing between 17-20 kg. The basal ration used was sugar cane pulp silage of urea which has the highest fermentability (experimental results 1) of the rumen fluid plus buffer with urea levels of 0.5% in comparison DM.dan concentrates 25%: 75% are prepared with CP 13.61 % and 63.71% TDN. There is interaction between the sardines oil with zinc on concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption, alkaline phosphatase activity, of ram fed the basal ration of sugar cane pulp that have been fermented and added urea. Interaction shown in, serum concentrations of PGE₂ (P<0.01) and absorption of zinc (P<0.01). At the level of sardines oil 1.5% and zinc 50 mg provides the best concentrations of prostaglandin-E₂, serum zinc levels, zinc absorption, alkaline phosphatase activity from the rams were given rations of sugar cane pulp that have been fermented and urea added.

Key words: sardines oil, zinc, prostaglandin-E₂, alkaline-phosphatase actvity, ram, sugar cane pulp